Regulatory

Part:BBa_K1401009:Design

Designed by: Kathleen Lewis   Group: iGEM14_BostonU   (2014-10-09)


Transcriptional Unit with pTet-pBad tandem promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 187
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 853
    Illegal AgeI site found at 965
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was created using the MoClo assembly method. The tandem promoters were created by combining two promoters together without any additional sequence in between.


Source

Tandem Promoter Reference Paper

References